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Cell-Free Production of a Therapeutic Protein: Further demonstration of efficacy of therapeutic proteins or vaccines derived from the leukocyte extracts will be determined pending optimization of product yields and concomitant purification of the protein as needed. Recovery and purification process development for monoclonal antibody production.

Introduction

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Recovery and purification process development for monoclonal antibody production. Point-of-care production of therapeutic proteins of good-manufacturing-practice quality. The results provide evidence that the leukocyte cell-free system is able to produce the recombinant proteins in a few hours. Approaches to miniaturize bioreactors to shift production to the point-of-care have been explored 3.

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